Direct-zol

R$0,00

Total RNA and small/miRNA from TRIzol without phase separation

  • Quick purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
  • Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.

Compatible with samples stored in TRIzol®, TRI-Reagent®, RNAzol®, QIAzol®, and all other acid-guanidinium-phenol reagents.

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Descrição

Overview

The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).

Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA (see below).

The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).

The entire procedure typically takes only 7 minutes.

Specifications

  • Size Range: RNA ≥17 nt
  • Equipment: Microcentrifuge, vortex
  • Yield: spin column, 100 µg RNA (binding capacity), ≥50 µl (elution volume)
  • Sample Source: Any sample stored and preserved in TRI Reagent, TRIzol or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
  • Compatibility: TRIzol, RNAzol, QIAzol, TriPure, TriSure and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent.
  • Sample Inactivation: TRI Reagent (provided with R2071, R2073) inhibits RNase activity and inactivates viruses and other infectious agents.

Technical Information & Protocol

Product SKU Binding Capacity Format Elution Volume Cells Tissue Protocol SDS
Direct-zol™ RNA Microprep R2061 10µg Spin-Column 6 µl ≤1×10 Cells ≤5 mg Tissue Protocol SDS (MSDS)
Direct-zol™ RNA Miniprep R2051 50 µg Spin-Column 25 µl ≤5×10 Cells ≤25 mg Tissue Protocol SDS (MSDS)
Direct-zol™ RNA Miniprep Plus R2071 100 µg Spin-Column 50 µl ≤1×10 Cells ≤50 mg Tissue Protocol SDS (MSDS)
Direct-zol™-96 R2055 10µg 96-Well Plate 10 µl ≤1×10 Cells ≤5 mg Tissue Protocol SDS (MSDS)
Direct-zol™-96 MagBead R2101 10µg Magnetic Beads 50 µl ≤1×10 Cells ≤5 mg Tissue Protocol SDS (MSDS)

⇓ Download of Data Sheet ⇓


 


MICROPREP
R2060 –     50 Preps – Without   TRI Reagent
R2061 –     50 Preps – With      TRI Reagent
R2062 –    200 Preps – Without   TRI Reagent
R2063 –    200 Preps – With      TRI Reagent


MINIPREP
R2050 –     50 Preps – Without   TRI Reagent
R2051 –     50 Preps – With      TRI Reagent
R2052 –    200 Preps – Without   TRI Reagent
R2053 –    200 Preps – With      TRI Reagent


MINIPREP PLUS
R2070  –    50 Preps – Without   TRI Reagent
R2071  –    50 Preps – With      TRI Reagent
R2072  –   200 Preps – Without   TRI Reagent
R2073  –   200 Preps – With      TRI Reagent
R2070T –    10 Preps – Without   TRI Reagent


96 – Well
R2054 – 2 x 96 Preps – Without   TRI Reagent
R2055 – 2 x 96 Preps – With      TRI Reagent
R2056 – 4 x 96 Preps – Without   TRI Reagent
R2057 – 4 x 96 Preps – With      TRI Reagent


MagBead
R2100 – 2 x 96 Preps – Without   TRI Reagent
R2101 – 2 x 96 Preps – With      TRI Reagent
R2102 – 4 x 96 Preps – Without   TRI Reagent
R2103 – 4 x 96 Preps – With      TRI Reagent
R2104 – 8 x 96 Preps – Without   TRI Reagent
R2105 – 8 x 96 Preps – With      TRI Reagent


 

Informação adicional

Kit

Micro Prep, Mini Prep, Mini Prep Plus, 96-well, Magbead

TRI-Reagente

Com, Sem

Prep Size

10 Preps, 50 Preps, 200 Preps, 2 x 96 Preps, 4 x 96 Preps, 8 x 96 Preps

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